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Eupolybothruscavernicolus Komerički & Stoev sp. n. (Chilopoda: Lithobiomorpha: Lithobiidae): the first eukaryotic species description combining transcriptomic, DNA barcoding and micro-CT imaging data

Identifieur interne : 000345 ( Main/Exploration ); précédent : 000344; suivant : 000346

Eupolybothruscavernicolus Komerički & Stoev sp. n. (Chilopoda: Lithobiomorpha: Lithobiidae): the first eukaryotic species description combining transcriptomic, DNA barcoding and micro-CT imaging data

Auteurs : Pavel Stoev [Bulgarie] ; Ana Komeri Ki [Croatie] ; Nesrine Akkari [Danemark] ; Shanlin Liu [République populaire de Chine] ; Xin Zhou [République populaire de Chine] ; Alexander M. Weigand [Allemagne, Croatie] ; Jeroen Hostens [Belgique] ; Christopher I. Hunter [République populaire de Chine] ; Scott C. Edmunds [République populaire de Chine] ; David Porco [France] ; Marzio Zapparoli [Italie] ; Teodor Georgiev [Bulgarie] ; Daniel Mietchen [Allemagne, Bulgarie] ; David Roberts [Royaume-Uni] ; Sarah Faulwetter [Grèce] ; Vincent Smith [Royaume-Uni] ; Lyubomir Penev [Bulgarie]

Source :

RBID : PMC:3964625

Abstract

We demonstrate how a classical taxonomic description of a new species can be enhanced by applying new generation molecular methods, and novel computing and imaging technologies. A cave-dwelling centipede, Eupolybothruscavernicolus Komerički & Stoev sp. n. (Chilopoda: Lithobiomorpha: Lithobiidae), found in a remote karst region in Knin, Croatia, is the first eukaryotic species for which, in addition to the traditional morphological description, we provide a fully sequenced transcriptome, a DNA barcode, detailed anatomical X-ray microtomography (micro-CT) scans, and a movie of the living specimen to document important traits of its ex-situ behaviour. By employing micro-CT scanning in a new species for the first time, we create a high-resolution morphological and anatomical dataset that allows virtual reconstructions of the specimen and subsequent interactive manipulation to test the recently introduced ‘cybertype’ notion. In addition, the transcriptome was recorded with a total of 67,785 scaffolds, having an average length of 812 bp and N50 of 1,448 bp (see GigaDB). Subsequent annotation of 22,866 scaffolds was conducted by tracing homologs against current available databases, including Nr, SwissProt and COG. This pilot project illustrates a workflow of producing, storing, publishing and disseminating large data sets associated with a description of a new taxon. All data have been deposited in publicly accessible repositories, such as GigaScience GigaDB, NCBI, BOLD, Morphbank and Morphosource, and the respective open licenses used ensure their accessibility and re-usability.


Url:
DOI: 10.3897/BDJ.1.e1013
PubMed: 24723752
PubMed Central: 3964625


Affiliations:


Links toward previous steps (curation, corpus...)


Le document en format XML

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Komerički & Stoev sp. n. (
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Komerički & Stoev sp. n. (
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): the first eukaryotic species description combining transcriptomic, DNA barcoding and micro-CT imaging data</title>
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<nlm:aff id="A9">
<addr-line>Université de Rouen - Laboratoire ECODIV, Mont Saint Aignan Cedex, France</addr-line>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Rouen - Laboratoire ECODIV, Mont Saint Aignan Cedex</wicri:regionArea>
<wicri:noRegion>Mont Saint Aignan Cedex</wicri:noRegion>
<wicri:noRegion>Mont Saint Aignan Cedex</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Zapparoli, Marzio" sort="Zapparoli, Marzio" uniqKey="Zapparoli M" first="Marzio" last="Zapparoli">Marzio Zapparoli</name>
<affiliation wicri:level="1">
<nlm:aff id="A10">
<addr-line>Università degli Studi della Tuscia, Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), Viterbo, Italy</addr-line>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Università degli Studi della Tuscia, Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), Viterbo</wicri:regionArea>
<wicri:noRegion>Viterbo</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Georgiev, Teodor" sort="Georgiev, Teodor" uniqKey="Georgiev T" first="Teodor" last="Georgiev">Teodor Georgiev</name>
<affiliation wicri:level="3">
<nlm:aff id="A2">
<addr-line>Pensoft Publishers, Sofia, Bulgaria</addr-line>
</nlm:aff>
<country xml:lang="fr">Bulgarie</country>
<wicri:regionArea>Pensoft Publishers, Sofia</wicri:regionArea>
<placeName>
<settlement type="city">Sofia</settlement>
<region nuts="2">Sofia-ville (oblast)</region>
</placeName>
</affiliation>
</author>
<author>
<name sortKey="Mietchen, Daniel" sort="Mietchen, Daniel" uniqKey="Mietchen D" first="Daniel" last="Mietchen">Daniel Mietchen</name>
<affiliation wicri:level="3">
<nlm:aff id="A11">
<addr-line>Museum für Naturkunde – Leibniz-Institut für Evolutions- und Biodiversitätsforschung, Berlin, Germany</addr-line>
</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Museum für Naturkunde – Leibniz-Institut für Evolutions- und Biodiversitätsforschung, Berlin</wicri:regionArea>
<placeName>
<region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3">
<nlm:aff id="A2">
<addr-line>Pensoft Publishers, Sofia, Bulgaria</addr-line>
</nlm:aff>
<country xml:lang="fr">Bulgarie</country>
<wicri:regionArea>Pensoft Publishers, Sofia</wicri:regionArea>
<placeName>
<settlement type="city">Sofia</settlement>
<region nuts="2">Sofia-ville (oblast)</region>
</placeName>
</affiliation>
</author>
<author>
<name sortKey="Roberts, David" sort="Roberts, David" uniqKey="Roberts D" first="David" last="Roberts">David Roberts</name>
<affiliation wicri:level="3">
<nlm:aff id="A12">
<addr-line>The Natural History Museum, London, United Kingdom</addr-line>
</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Natural History Museum, London</wicri:regionArea>
<placeName>
<settlement type="city">Londres</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Grand Londres</region>
</placeName>
</affiliation>
</author>
<author>
<name sortKey="Faulwetter, Sarah" sort="Faulwetter, Sarah" uniqKey="Faulwetter S" first="Sarah" last="Faulwetter">Sarah Faulwetter</name>
<affiliation wicri:level="1">
<nlm:aff id="A13">
<addr-line>National and Kapodestrian University of Athens, Athens, Greece</addr-line>
</nlm:aff>
<country xml:lang="fr">Grèce</country>
<wicri:regionArea>National and Kapodestrian University of Athens, Athens</wicri:regionArea>
<wicri:noRegion>Athens</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1">
<nlm:aff id="A14">
<addr-line>Hellenic Centre for Marine Research, Heraklion, Greece</addr-line>
</nlm:aff>
<country xml:lang="fr">Grèce</country>
<wicri:regionArea>Hellenic Centre for Marine Research, Heraklion</wicri:regionArea>
<wicri:noRegion>Heraklion</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Smith, Vincent" sort="Smith, Vincent" uniqKey="Smith V" first="Vincent" last="Smith">Vincent Smith</name>
<affiliation wicri:level="3">
<nlm:aff id="A12">
<addr-line>The Natural History Museum, London, United Kingdom</addr-line>
</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>The Natural History Museum, London</wicri:regionArea>
<placeName>
<settlement type="city">Londres</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Grand Londres</region>
</placeName>
</affiliation>
</author>
<author>
<name sortKey="Penev, Lyubomir" sort="Penev, Lyubomir" uniqKey="Penev L" first="Lyubomir" last="Penev">Lyubomir Penev</name>
<affiliation wicri:level="3">
<nlm:aff id="A15">
<addr-line>Institute of Biodiversity & Ecosystem Research - Bulgarian Academy of Sciences and Pensoft Publishers, Sofia, Bulgaria</addr-line>
</nlm:aff>
<country xml:lang="fr">Bulgarie</country>
<wicri:regionArea>Institute of Biodiversity & Ecosystem Research - Bulgarian Academy of Sciences and Pensoft Publishers, Sofia</wicri:regionArea>
<placeName>
<settlement type="city">Sofia</settlement>
<region nuts="2">Sofia-ville (oblast)</region>
</placeName>
</affiliation>
</author>
</analytic>
<series>
<title level="j">Biodiversity Data Journal</title>
<idno type="ISSN">1314-2836</idno>
<idno type="eISSN">1314-2828</idno>
<imprint>
<date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc>
<textClass></textClass>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">
<label>Abstract</label>
<p>We demonstrate how a classical taxonomic description of a new species can be enhanced by applying new generation molecular methods, and novel computing and imaging technologies. A cave-dwelling centipede,
<italic>
<named-content content-type="taxon-name">
<named-content content-type="genus">Eupolybothrus</named-content>
<named-content content-type="species">cavernicolus</named-content>
</named-content>
</italic>
Komerički & Stoev sp. n. (
<named-content content-type="taxon-name">
<named-content content-type="classis">Chilopoda</named-content>
</named-content>
:
<named-content content-type="taxon-name">
<named-content content-type="ordo">Lithobiomorpha</named-content>
</named-content>
:
<named-content content-type="taxon-name">
<named-content content-type="familia">Lithobiidae</named-content>
</named-content>
), found in a remote karst region in Knin, Croatia, is the first eukaryotic species for which, in addition to the traditional morphological description, we provide a fully sequenced transcriptome, a DNA barcode, detailed anatomical X-ray microtomography (micro-CT) scans, and a movie of the living specimen to document important traits of its ex-situ behaviour. By employing micro-CT scanning in a new species for the first time, we create a high-resolution morphological and anatomical dataset that allows virtual reconstructions of the specimen and subsequent interactive manipulation to test the recently introduced ‘cybertype’ notion. In addition, the transcriptome was recorded with a total of 67,785 scaffolds, having an average length of 812 bp and N50 of 1,448 bp (see GigaDB). Subsequent annotation of 22,866 scaffolds was conducted by tracing homologs against current available databases, including Nr, SwissProt and COG. This pilot project illustrates a workflow of producing, storing, publishing and disseminating large data sets associated with a description of a new taxon. All data have been deposited in publicly accessible repositories, such as GigaScience GigaDB, NCBI, BOLD, Morphbank and Morphosource, and the respective open licenses used ensure their accessibility and re-usability.</p>
</div>
</front>
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<name sortKey="Zapparoli, M" uniqKey="Zapparoli M">M Zapparoli</name>
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<name sortKey="Prohaska, Steffen" uniqKey="Prohaska S">Steffen Prohaska</name>
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<li>République populaire de Chine</li>
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<li>Berlin</li>
<li>Copenhague</li>
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<name sortKey="Stoev, Pavel" sort="Stoev, Pavel" uniqKey="Stoev P" first="Pavel" last="Stoev">Pavel Stoev</name>
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<name sortKey="Weigand, Alexander M" sort="Weigand, Alexander M" uniqKey="Weigand A" first="Alexander M." last="Weigand">Alexander M. Weigand</name>
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<name sortKey="Akkari, Nesrine" sort="Akkari, Nesrine" uniqKey="Akkari N" first="Nesrine" last="Akkari">Nesrine Akkari</name>
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<name sortKey="Liu, Shanlin" sort="Liu, Shanlin" uniqKey="Liu S" first="Shanlin" last="Liu">Shanlin Liu</name>
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<name sortKey="Hunter, Christopher I" sort="Hunter, Christopher I" uniqKey="Hunter C" first="Christopher I." last="Hunter">Christopher I. Hunter</name>
<name sortKey="Zhou, Xin" sort="Zhou, Xin" uniqKey="Zhou X" first="Xin" last="Zhou">Xin Zhou</name>
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<name sortKey="Hostens, Jeroen" sort="Hostens, Jeroen" uniqKey="Hostens J" first="Jeroen" last="Hostens">Jeroen Hostens</name>
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<name sortKey="Porco, David" sort="Porco, David" uniqKey="Porco D" first="David" last="Porco">David Porco</name>
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<noRegion>
<name sortKey="Zapparoli, Marzio" sort="Zapparoli, Marzio" uniqKey="Zapparoli M" first="Marzio" last="Zapparoli">Marzio Zapparoli</name>
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<country name="Royaume-Uni">
<region name="Angleterre">
<name sortKey="Roberts, David" sort="Roberts, David" uniqKey="Roberts D" first="David" last="Roberts">David Roberts</name>
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<name sortKey="Smith, Vincent" sort="Smith, Vincent" uniqKey="Smith V" first="Vincent" last="Smith">Vincent Smith</name>
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<country name="Grèce">
<noRegion>
<name sortKey="Faulwetter, Sarah" sort="Faulwetter, Sarah" uniqKey="Faulwetter S" first="Sarah" last="Faulwetter">Sarah Faulwetter</name>
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<name sortKey="Faulwetter, Sarah" sort="Faulwetter, Sarah" uniqKey="Faulwetter S" first="Sarah" last="Faulwetter">Sarah Faulwetter</name>
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</record>

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